DNA

Part:BBa_J119005:Design

Designed by: Caleb J. Carr   Group: Eckdahl Lab   (2011-07-21)


BsmBI-RBS-RFP1-BsaI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 381


Design Notes

It was quite difficult to synthesize this half-edge (first half of the gene to be used in solving a path problem). This is NOT a BioBrick Part, it has been produced for the purpose of putting together and solving HPP (Hamiltonian Path Problems) by adding its second half (and others) to competent cells for re-assembling.


Source

PCR Amplification of the RFP gene template.

References