DNA
Part:BBa_J119005:Design
Designed by: Caleb J. Carr Group: Eckdahl Lab (2011-07-21)
BsmBI-RBS-RFP1-BsaI
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 387 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 387 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 387 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 387 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 381
Design Notes
It was quite difficult to synthesize this half-edge (first half of the gene to be used in solving a path problem). This is NOT a BioBrick Part, it has been produced for the purpose of putting together and solving HPP (Hamiltonian Path Problems) by adding its second half (and others) to competent cells for re-assembling.
Source
PCR Amplification of the RFP gene template.